Question#1
A. Forward primer (5’ -> 3’): TGCTCCTGCCGAGAAAGTAT
EMBOSS_001 1
---------------atgcgcaaaggcgaagaactgtttaccggcgtggt 35
Beta-2-adrene 1951 GCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCG 2000
|||.||.|||||.||.|||||.|||||.||.||||||||.||.||.||||
EMBOSS_001 36
gccgattctggtggaactggatggcgatgtgaacggccataaatttagcg 85
Beta-2-adrene 2001 TGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAG 2050
||..||||||.|||||.||||||||.|||.|||||||.|||||||||||.
EMBOSS_001 86
tgagcggcgaaggcgaaggcgatgcgaccaacggcaaactgaccctgaaa 135
%
G-C
is 52.12%
%
content:
Tm = 59.68
Ta
= 50
B. Reverse primer (5’ -> 3’): GAAATCTCGTGATGGCAGGT
%
G-C
is 50% content: Tm =60.08
Ta
= 46
Question#2
Beta-2-adrene 2201 CGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGT 2250
||||||||...|||.||.||.||.||||.|||.||.||||||||.||.||
EMBOSS_001 286 cgcaccattagctttaaagatgatggcacctataaaacccgcgcggaagt 335
Beta-2-adrene 2251 GAAGTTCGAGGGCGACACCCTGGTGAACCGCATcGAGCTGAAGGGCATCG 2300
|||.||.||.|||||.|||||||||||||||||.||.|||||.|||||.|
EMBOSS_001 336 gaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattg 385
Beta-2-adrene 2301 ACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTAC 2350
|.||.||.||.||.||||||||.|||||.||.||.|||||.||.||||..
EMBOSS_001 386 attttaaagaagatggcaacattctgggccataaactggaatataacttt 435
Reverse primer (5’ -> 3’): GAAGTTTCCTGTTGGCAGGT
%
G-C
is 49% content: Tm = 63
Ta
= 61
Question#3
Beta-2-adrene 2351 AACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAA 2400
||||||||.|||||.|||||.|..||.||.||.|||||.||||||||.||
EMBOSS_001 436 aacagccataacgtgtatattaccgcggataaacagaaaaacggcattaa 485
Beta-2-adrene 2401 GGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCG 2450
.|.||||||.||.||.|||||.|||.|.||.||.||||||||||||||.|
EMBOSS_001 486 agcgaactttaaaattcgccataacgtggaagatggcagcgtgcagctgg 535
Beta-2-adrene 2451 CCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTG 2500
|.||.||.||.||||||||||||||.||.|||||.|||||.|||||||||
A. Forward primer (5’ -> 3’): TGTTCCTACCGAGGCCGTAT
%
G-C
is 50%
content: Tm = 45.75
Ta
= 49
B. Reverse primer (5’ -> 3’): GACCTCTCGTCGTAACACAT
%
G-C
is 56% content: Tm = 56.32
Ta
= 61.20
C. Tm
value of one of my primers is 45.75, and another one is 63. The PCR product
size is 282 and GC %age is 56. The PCR profile was needed to be modified in
order to accommodate new primers. Only minor change the PCR amplification
reaction is required.
Question#4
The appearance of the four
types of samples generated in this experiment has mostly been dark. For
instance, the Phase Contrast stage of the Untreated part was found to be of
gray color with white, rounded-circles inside it. The cellular distribution has
been uneven; in some places, cells were more visible than others.
